Heat shock proteins of higher plants

Abstract

The pattern of protein synthesis changes rapidly and dramatically when the growth temperature of soybean seedling tissue is increased from 28 degrees C (normal) to about 40 degrees C (heat shock). The synthesis of normal proteins is greatly decreased and a new set of proteins, "heat shock proteins," is induced. The heat shock proteins of soybean consist of 10 new bands on one-dimensional NaDodSO(4) gels; a more complex pattern is observed on two-dimensional gels. When the tissue is returned to 28 degrees C after 4 hr at 40 degrees C, there is progressive decline in the synthesis of heat shock proteins and reappearance of a normal pattern of synthesis by 3 or 4 hr. In vitro translation of poly(A)(+)RNAs isolated from tissues grown at 28 and 40 degrees C shows that the heat shock proteins are translated from a new set of mRNAs induced at 40 degrees C; furthermore, the abundant class mRNAs for many of the normal proteins persist even though they are translated weakly (or not at all) in vivo at 40 or 42.5 degrees C. The heat shock response in soybean appears similar to the much-studied heat shock phenomenon in Drosophila.