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. 1982 Sep;79(18):5494-8.
doi: 10.1073/pnas.79.18.5494.

Reconstruction of the chloroplast noncyclic electron transport pathway from water to NADP with three integral protein complexes

E Lam  1 , R Malkin
Affiliations

Reconstruction of the chloroplast noncyclic electron transport pathway from water to NADP with three integral protein complexes

E Lam et al. Proc Natl Acad Sci U S A. 1982 Sep.

Abstract

RECONSTRUCTION OF PHOTOSYNTHETIC NONCYCLIC ELECTRON TRANSPORT FROM WATER TO NADP HAS BEEN ACCOMPLISHED BY USING THREE INTEGRAL PROTEIN COMPLEXES ISOLATED FROM CHLOROPLAST THYLAKOID MEMBRANES: photosystems I and II and the cytochrome b(6)-f complex. This system shows an absolute dependence on the presence of all three protein complexes for NADP reduction, in addition to plastocyanin, ferredoxin, and ferredoxin-NADP reductase. The reconstructed system was found to be sensitive to low concentrations of known inhibitors of noncyclic electron transport. Depletion of the Rieske iron-sulfur center and bound plastoquinone from the cytochrome b(6)-f complex resulted in an inhibition of the photoreduction of NADP.

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