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. 1984 Apr;81(8):2406-10.
doi: 10.1073/pnas.81.8.2406.

Chemically defined media for cultivation of Naegleria gruberi

C Fulton  1 , C Webster, J S Wu
Affiliations

Chemically defined media for cultivation of Naegleria gruberi

C Fulton et al. Proc Natl Acad Sci U S A. 1984 Apr.

Abstract

Cultivation of amebae of the axenic strain of Naegleria gruberi, NEG-M, was achieved in media consisting entirely of chemically defined components. A complete medium that contains 31 components allows growth with yields up to 5 x 10(6) amebae per ml. A minimal medium gives lower yields but defines 22 components that are essential for continuous cultivation: 11 amino acids, 6 vitamins, hematin, guanosine, D-glucose, Mg(2+), and inorganic phosphate. These media allow precise studies of the metabolism and differentiation of this unusual eukaryote.

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