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. 1985 Oct;82(20):6899-902.
doi: 10.1073/pnas.82.20.6899.

Identification of a high molecular weight polypeptide that may be part of the circadian clockwork in Acetabularia

R Hartwig  1 , M Schweiger, R Schweiger, H G Schweiger
Affiliations

Identification of a high molecular weight polypeptide that may be part of the circadian clockwork in Acetabularia

R Hartwig et al. Proc Natl Acad Sci U S A. 1985 Oct.

Abstract

In the chloroplast fraction of the unicellular and uninucleate green alga Acetabularia, we have detected a M(r) approximately 230,000 protein (p230) whose synthesis exhibits a pronounced endogenous diurnal rhythm. As judged by scanning densitometry of fluorographs of NaDodSO(4)/polyacrylamide gels, the synthesis of other proteins in the same fraction was independent of the time in the cycle. The incorporation of [(35)S]methionine into p230 was completely inhibited by cycloheximide, whereas chloramphenicol had no effect. This strongly suggests that p230 is translated on 80S ribosomes. Eighthour periods of exposure to cycloheximide produced a shift in the phase of the oscillation of p230 synthesis. The results are consistent with the hypothesis that p230 is essential for expression of circadian rhythms in Acetabularia.

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