Reconstitution of chlorophyll a/b light-harvesting complexes: Xanthophyll-dependent assembly and energy transfer
- PMID: 16593794
- PMCID: PMC304159
- DOI: 10.1073/pnas.84.1.146
Reconstitution of chlorophyll a/b light-harvesting complexes: Xanthophyll-dependent assembly and energy transfer
Abstract
A method for in vitro reconstitution of the chlorophyll a/b light-harvesting complex from LiDodSO(4)/heat-denatured or acetone-extracted photosynthetic membranes has been developed. Characterization of the minimum components necessary for the functional organization of pigments in these membrane complexes reveals that xanthophylls are essential structural components.
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