Specific binding of a fungal glucan phytoalexin elicitor to membrane fractions from soybean Glycine max

Abstract

Treatment of soybean tissues with elicitors results in the production of phytoalexins, one of a number of inducible plant defense reactions against microbial infections. The present study uses a beta-1,3-[(3)H]glucan elicitor fraction from Phytophthora megasperma f. sp. glycinea, a fungal pathogen of soybean, to identify putative elicitor targets in soybean tissues. Use of the radiolabeled elicitor disclosed saturable high-affinity elicitor binding site(s) in membrane fractions of soybean roots. Highest binding activity is associated with a plasma membrane-enriched fraction. The apparent K(d) value for beta-glucan elicitor binding is approximately 0.2 x 10(-6) M and the maximum number of binding sites is 0.5 pmol per mg of protein. Competition studies with the [(3)H]glucan elicitor and a number of polysaccharides demonstrate that only polysaccharides of a branched beta-glucan type effectively displace the radiolabeled ligand from membrane binding. Differential displacing activity of the glucans on P. megasperma elicitor binding corresponds closely to their respective ability to elicit phytoalexin production in a cotyledon bioassay.