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. 1991 Nov 25;19(22):6155-61.
doi: 10.1093/nar/19.22.6155.

High level expression and purification of herpes simplex virus type 1 immediate early polypeptide Vmw110

R D Everett  1 , A Orr, M Elliott
Affiliations
Free PMC article

High level expression and purification of herpes simplex virus type 1 immediate early polypeptide Vmw110

R D Everett et al. Nucleic Acids Res. .
Free PMC article

Abstract

Herpes simplex virus type 1 (HSV-1) encodes five immediate early (IE) polypeptides. This paper reports the construction of a baculovirus vector which expresses large amounts of Vmw110, the product of IE gene 1. The expressed protein has been purified to near homogeneity and has a mobility on SDS polyacrylamide gels identical to that of Vmw110 produced during HSV-1 infection. Characterisation of its properties indicated that it forms dimers and perhaps higher order oligomers in solution and that the purified protein binds to both single stranded and double stranded calf thymus DNA cellulose columns. However, filter binding experiments were unable to detect any stable association of Vmw110 with DNA in solution.

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References

    1. Cell. 1991 Feb 8;64(3):483-4 - PubMed
    1. Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 - PubMed
    1. J Gen Virol. 1991 Mar;72 ( Pt 3):651-9 - PubMed
    1. Cell. 1991 Jun 14;65(6):1063-72 - PubMed
    1. Cell. 1991 Jun 14;65(6):1053-61 - PubMed