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. 2006 Apr;44(4):1567-70.
doi: 10.1128/JCM.44.4.1567-1570.2006.

Rapid identification of Enterococcus hirae and Enterococcus durans by PCR and detection of a homologue of the E. hirae mur-2 Gene in E. durans

Cesar A Arias  1 , Beatriz Robredo, Kavindra V Singh, Carmen Torres, Diana Panesso, Barbara E Murray
Affiliations

Rapid identification of Enterococcus hirae and Enterococcus durans by PCR and detection of a homologue of the E. hirae mur-2 Gene in E. durans

Cesar A Arias et al. J Clin Microbiol. 2006 Apr.

Abstract

During an evaluation of PCR for identification of isolates of Enterococcus hirae, a homologue with 82% identity to E. hirae mur-2 was identified in Enterococcus durans and was named mur-2(ed). PCR using primers for two genes (copY and murG) of E. hirae strains showed amplification with E. hirae strains only. PCR (under high-stringency conditions) with primers for the mur-2(ed) gene gave the expected amplification product only with E. durans strains. A combination of murG and mur-2(ed) primers in a multiplex PCR assay differentiated E. hirae from E. durans in all cases. PCR using these primers appears to be a rapid alternative for identification of E. hirae and E. durans isolates.

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Figures

FIG. 1.
FIG. 1.
Multiplex PCR of Enterococcus isolates using murG and mur2ed primers under low-stringency conditions with total DNA as the template. Lane 1, molecular weight marker; lane 2, E. durans ATCC 49479 (identified as E. hirae in this work); lanes 3 and 4, E. hirae clinical isolates TX2817 and TX0302, respectively; lane 5, E. durans ATCC 6056; lane 6, E. gallinarum clinical isolate; lane 7, E. faecalis OG1RF; lane 8, E. faecium TX0016.
See this image and copyright information in PMC

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