doi: 10.1016/s0006-291x(05)81439-1.
Expression of a biologically active ovine trophoblastic interferon using a baculovirus expression system
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- PMID: 1659817
- DOI: 10.1016/s0006-291x(05)81439-1
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Expression of a biologically active ovine trophoblastic interferon using a baculovirus expression system
Biochem Biophys Res Commun.
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Abstract
Ovine trophoblast protein (oTP) an embryonic interferon, which plays a key role in maternal recognition of pregnancy, has been expressed in insect cells using a baculovirus expression system. A cDNA coding for oTP was inserted downstream of the strong polyhedrin promoter. Cells infected with recombinant virus produced biologically active oTP and greater than 90% was secreted into the culture medium during infection. High amount of antiviral activity were produced (up to 5 x 10(5) IU per ml of culture medium). Recombinant oTP (roTP) was purified by immunoaffinity chromatography and found to be identical to authentic oTP with respect to molecular mass and N-terminal amino acid sequence.
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