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. 2003:1:e008.
doi: 10.1621/nrs.01008. Epub 2003 Sep 10.

RNAi technology and its use in studying the function of nuclear receptors and coregulators

Hai-Jun Zhou  1 , Sophia Y Tsai, Ming-Jer Tsai
Affiliations

RNAi technology and its use in studying the function of nuclear receptors and coregulators

Hai-Jun Zhou et al. Nucl Recept Signal. 2003.

Abstract

Until just a few years ago, RNA interference (RNAi) technology was restricted to the research fields of plants, C. elegans or Drosophila. The discovery of gene silencing by in vitro synthesized double-stranded RNA (dsRNA) in mammalian cells has made the use of RNAi possible in nearly the entire life science kingdom. DNA vectors delivering small interfering RNA (siRNA) directed by polymerase III or polymerase II promoters to persistently inhibit target genes expression have extended this technology to study in vivo function of these genes. Recently, RNAi has been used as a powerful tool in the functional analysis of nuclear receptors and their coregulators. This short review will cover studies in this area.

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References

    1. Baglioni C., Nilsen T. W. Mechanisms of antiviral action of interferon. Interferon. 1983;5:23–42. - PubMed
    1. Brummelkamp T. R., Bernards R., Agami R. A system for stable expression of short interfering RNAs in mammalian cells. Science. 2002;296:550–3. - PubMed
    1. Caplen N. J., Parrish S., Imani F., Fire A., Morgan R. A. Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Proc Natl Acad Sci U S A. 2001;98:9742–7. - PMC - PubMed
    1. Cavarretta I. T., Mukopadhyay R., Lonard D. M., Cowsert L. M., Bennett C. F., O'Malley B. W., Smith C. L. Reduction of coactivator expression by antisense oligodeoxynucleotides inhibits ERalpha transcriptional activity and MCF-7 proliferation. Mol Endocrinol. 2002;16:253–70. - PubMed
    1. Debes J. D., Schmidt L. J., Huang H., Tindall D. J. p300 mediates androgen-independent transactivation of the androgen receptor by interleukin 6. Cancer Res. 2002;62:5632–6. - PubMed

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