Molecularly defined adult-type hypolactasia in school-aged children with a previous history of cow's milk allergy

Abstract

Aim: To assess the role of lactase non-persistence/persistence in school-aged children and their milk-related symptoms.

Methods: The genotypes for the C/T(-13910) variant associated with lactase non-persistence/ persistence were determined using PCR-minisequencing in a group of 172 children with a mean age of 8.6 years (SE = 0.02, 93 boys) participating in a follow-up study for cow's milk allergy. The parents were asked to assess their children's milk consumption and abdominal symptoms.

Results: The presence of allergy to cow's milk was not associated with the C/C(-13910) genotype related with a decline of lactase enzyme activity during childhood (lactase non-persistence). The frequency of the C/C(-13910) genotype (16%) was similar to published figures for the prevalence of adult-type hypolactasia in Finland. The majority of the children (90%) in this series consumed milk but 26% of their families suspected that their children had milk-related symptoms. Forty-eight percent of the children with the C/C(-13910) genotype did not drink milk at all or consumed a low lactose containing diet prior to the genotyping (P < 0.004 when compared to the other genotypes).

Conclusion: Analysis of the C/T(-13910) polymorphism is an easy and reliable method for excluding adult-type hypolactasia in children with milk-related symptoms. Genotyping for this variant can be used to advise diets for children with a previous history of cow's milk allergy.