Neuroprotective effects of neuregulin-1 in rat models of focal cerebral ischemia
- PMID: 16616052
- DOI: 10.1016/j.brainres.2006.03.007
Neuroprotective effects of neuregulin-1 in rat models of focal cerebral ischemia
Abstract
The purpose of this study was to investigate the therapeutic efficacy and mechanism of recombinant human NRG-1 to attenuate ischemia/reperfusion brain injury. NRG-1(3.0 ng/kg) was applied intravascularly 10 min before middle cerebral artery occlusion (MCAO) and then focal cerebral ischemia for 90 min and reperfusion for 24 h. The rats were scored post-reperfusion for neurological deficits and infarct volume in the brain was assessed by 2,3,5-triphenyltetrazolium chloride(TTC). Apoptosis was evaluated by TUNEL staining. Reverse transcription polymerase chain reaction (RT-PCR) was used to measure changes of caspase-3 mRNA. The level of TNF-alpha was determined using enzyme-linked immunosorbent assay (ELISA). Our results demonstrated that recombinant human NRG-1 could reduce cerebral infarct volume by about 71% (P < 0.05) and TUNEL positive cells when given immediately before MCAO, and improved behavior of animals. Furthermore, we also showed that NRG-1 could also decrease the expression of caspase-3 mRNA and production of TNF-alpha protein. These data suggest that pre-administration of NRG-1 attenuates cerebral ischemia and reperfusion injury. This protective effect may be involved in the inhibition of caspase-3 and TNF-alpha.
Similar articles
-
Administration of N-acetylcysteine after focal cerebral ischemia protects brain and reduces inflammation in a rat model of experimental stroke.J Neurosci Res. 2004 May 15;76(4):519-27. doi: 10.1002/jnr.20087. J Neurosci Res. 2004. PMID: 15114624
-
Thalidomide protects against ischemic neuronal damage induced by focal cerebral ischemia in mice.Neuroscience. 2009 Mar 17;159(2):760-9. doi: 10.1016/j.neuroscience.2008.12.043. Epub 2009 Jan 1. Neuroscience. 2009. PMID: 19166916
-
The Neuroprotection of puerarin against cerebral ischemia is associated with the prevention of apoptosis in rats.Planta Med. 2005 Jul;71(7):585-91. doi: 10.1055/s-2005-871261. Planta Med. 2005. PMID: 16041641
-
[On the quantitative analysis of focal ischemic cerebral infarction by TTC staining].Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2009 Dec;26(6):1363-6. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2009. PMID: 20095504 Review. Chinese.
-
[Neuroprotective effects of magnesium on cerebral ischemia and cerebral contusion].Clin Calcium. 2004 Aug;14(8):60-4. Clin Calcium. 2004. PMID: 15577098 Review. Japanese.
Cited by
-
Prediction and Validation of Hub Genes Related to Major Depressive Disorder Based on Co-expression Network Analysis.J Mol Neurosci. 2024 Jan 10;74(1):8. doi: 10.1007/s12031-023-02172-5. J Mol Neurosci. 2024. PMID: 38198075
-
Impact of neuregulin-1 on the pathophysiology of schizophrenia in human post-mortem studies.Eur Arch Psychiatry Clin Neurosci. 2008 Nov;258 Suppl 5:35-9. doi: 10.1007/s00406-008-5019-x. Eur Arch Psychiatry Clin Neurosci. 2008. PMID: 18985292 Review.
-
Neuregulin-1 attenuates cognitive function impairments in a transgenic mouse model of Alzheimer's disease.Cell Death Dis. 2016 Feb 25;7(2):e2117. doi: 10.1038/cddis.2016.30. Cell Death Dis. 2016. PMID: 26913607 Free PMC article.
-
Neuregulin-1 inhibits CoCl2-induced upregulation of excitatory amino acid carrier 1 expression and oxidative stress in SH-SY5Y cells and the hippocampus of mice.Mol Brain. 2020 Nov 13;13(1):153. doi: 10.1186/s13041-020-00686-2. Mol Brain. 2020. PMID: 33187547 Free PMC article.
-
Transcriptomic analysis of neuregulin-1 regulated genes following ischemic stroke by computational identification of promoter binding sites: A role for the ETS-1 transcription factor.PLoS One. 2018 Jun 1;13(6):e0197092. doi: 10.1371/journal.pone.0197092. eCollection 2018. PLoS One. 2018. PMID: 29856744 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials