Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2006 Aug;8(4):254-60.
doi: 10.1016/j.jfms.2006.01.006. Epub 2006 Apr 17.

Platelet-bound antibodies detected by a flow cytometric assay in cats with thrombocytopenia

Barbara Kohn  1 , Tanja Linden, Wolfgang Leibold
Affiliations
Comparative Study

Platelet-bound antibodies detected by a flow cytometric assay in cats with thrombocytopenia

Barbara Kohn et al. J Feline Med Surg. 2006 Aug.

Abstract

In cats, primary or secondary immune-mediated thrombocytopenia have rarely been described or characterised. The objective of this study was to determine platelet-bound antibodies (PBA) by a flow cytometric assay in both healthy and thrombocytopenic cats. Direct PBA testing was performed in 42 thrombocytopenic cats (platelet counts 6-179 x 10(9)/l, median 56 x 10(9)/l). Of these 42 cats, 19 had positive PBA test results, 17 of which were considered to have secondary immune-mediated thrombocytopenia (sITP). Underlying diseases included fat necroses (four cases), feline infectious peritonitis (three), feline leukaemia virus (two) or feline immunodeficiency virus (two) infections, lymphoma (two), leukaemia (one), hepatitis (one), pyelonephritis (one), or hyperthyroidism (one). In two cats, no underlying disease was found suggesting a primary immune-mediated thrombocytopenia (pITP). The PBA test was negative in 23 cats diagnosed with varying underlying diseases and in 47 healthy control cats with platelet values within the reference range. Only seven of the 42 cats with thrombocytopenia (platelet count 10-57 x 10(9)/l, median 34 x 10(9)/l) had spontaneous bleeding. This study suggests that immune-mediated destruction of platelets might be an important pathological mechanism for feline thrombocytopenia caused by various underlying diseases. In cats, pITP appears to be rarely diagnosed.

PubMed Disclaimer

Figures

Fig 1
Fig 1
Forty-two cats with thrombocytopenia; number of platelets in cats with and without spontaneous bleeding. The number of platelets was significantly lower in cats with spontaneous bleeding than in cats without spontaneous bleeding (P=0.018).
See this image and copyright information in PMC

References

    1. Axthelm M.K., Krakowa S. Canine distemper virus-induced thrombocytopenia, American Journal of Veterinary Research 48, 1987, 1269–1275. - PubMed
    1. Baldwin C.J., Cowell R.L. Thrombocytopenia. August J.R. Consultations in Feline Internal Medicine, 4th edn, 2001, WB Saunders Company: Philadelphia, 468–478.
    1. Bithell T.C. Thrombotic thrombocytopenic purpura and other forms of non-immunologic platelet destruction. Lee G.R., Bithell T.C., Foerster J., Athens J.W., Lukens J.N. Wintrobe's Clinical Hematology, 9th edn, 1993, Lea and Fiebiger: Philadelphia, 1356–1362.
    1. Cain G.R., Cain J.L., Turrel J.M., Theilen G., Jain N.C. Immune-mediated hemolytic anemia and thrombocytopenia in a cat after bone marrow transplantation, Veterinary Pathology 25, 1988, 161–162. - PubMed
    1. Campbell K.L., George J.W., Greene C.E. Application of the enzyme-linked immunosorbent assay for the detection of platelet antibodies in dogs, American Journal of Veterinary Research 45, 1984, 2561–2564. - PubMed

Publication types