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. 2006 Jan-Feb;42(1-2):1-3.
doi: 10.1007/s11626-006-0002-1.

A new cell line from the wax moth Galleria mellonella Linne (Lepidoptera: Pyralididae)

Daisuke Eguchi  1 , Kikuo Iwabuchi
Affiliations

A new cell line from the wax moth Galleria mellonella Linne (Lepidoptera: Pyralididae)

Daisuke Eguchi et al. In Vitro Cell Dev Biol Anim. 2006 Jan-Feb.

Abstract

A cell line derived from the larval-fat body tissues of the wax moth, Galleria mellonella Linne, was established in MGM-450 medium. The cells grew in suspension and were mainly spherical in shape. Population doubling time was between 1.4 and 1.7 d over a range of 15 to 35 degrees C, and the maximum growth rate was at 25 degrees C. The chromosome number ranged from 70-239, with a mode of 170. The cells were sensitive to 20-hydroxyecdysone, which stimulated their growth and induced morphological changes. The cell line was designated GaMe-LF1.

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