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. 2006 Jan 10;86(2):128-32.

[Research of RhD protein in Rh blood group Del phenotype]

[Article in Chinese]
Wen Xiong  1 , Chao-peng Shao, Xue-mei Li, Yi-yan Zhou
Affiliations
  • PMID: 16620722

[Research of RhD protein in Rh blood group Del phenotype]

[Article in Chinese]
Wen Xiong et al. Zhonghua Yi Xue Za Zhi. .

Abstract

Objective: To study the expression of RhD protein in Rh blood group Del phenotype.

Methods: Peripheral blood samples were collected from 3 voluntary blood donors with different Rh phenotypes: CCD(el)D(el)ee, CcD(el)dee, and CcD(el)D(el)ee, and 4 controls: 2 RhD positive with the phenotypes of CCDDee and CcDDEe, and 2 RhD negative with the phenotypes: Ccddee and ccddee. Reverse transcriptase PCR (RT-PCR) and cDNA sequencing were used to detect the RhD mRNA and the expression of Del protein.

Results: The Del individuals all showed complicated RhD mRNA isolations, including 6 transcripts: isoforms with exons 7-9 spliced, exons 7 and 9 spliced, exons 8 and 9 spliced, exon 9 spliced and 2 long transcripts with exons 8 and 9 spliced or exon 9 spliced, but containing an additional segment of sequence from RHD intron 7 (917-1086, GenBank AB035194), those transcripts all being out of exon 9 and the Del9 transcript being the most similar to the normal RhD mRNA.

Conclusion: Normal RhD mRNA does not exist in the Del blood group that does not code normal RhD protein.

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