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Case Reports
. 1991 Dec 15;88(24):11315-9.
doi: 10.1073/pnas.88.24.11315.

Inactivation of the cholinesterase gene by Alu insertion: possible mechanism for human gene transposition

K Muratani  1 , T Hada, Y Yamamoto, T Kaneko, Y Shigeto, T Ohue, J Furuyama, K Higashino
Affiliations
Case Reports

Inactivation of the cholinesterase gene by Alu insertion: possible mechanism for human gene transposition

K Muratani et al. Proc Natl Acad Sci U S A. .

Abstract

The human cholinesterase (ChE) gene from a patient with acholinesterasemia was cloned and analyzed. By using ChE cDNA as a probe, four independent clones were isolated from a genomic library constructed from the patient's DNA. Sequencing analysis of all of the four clones revealed that exon 2 of the ChE gene was disrupted by a 342-base-pair (bp) insertion of Alu element, including a poly(A) tract of 38 bp, which showed 93% sequence homology with a current type of human Alu consensus sequence. Southern blot analysis showed that the Alu insertion occurred in both alleles of the patient and was inherited in the patient's family. This Alu insertion was flanked by 15-bp of target site duplication in exon 2 corresponding to positions 1062-1076 of ChE cDNA, indicating that an Alu element could have been integrated by retrotransposition. Thus, this case provides an important clue to the mechanism of inactivation of a gene by integration of a retrotransposon.

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