Intranasal infection of ferrets (Mustela putorius furo) with canine parainfluenza virus
- PMID: 1663681
- DOI: 10.1111/j.1439-0450.1991.tb00904.x
Intranasal infection of ferrets (Mustela putorius furo) with canine parainfluenza virus
Abstract
Immunocompetent and cyclophosphamide-immunosuppressed ferrets were intranasally infected with canine parainfluenza virus (CPIV) and observed for clinical signs, histopathologic lesions, the immunocytochemical demonstration of CPIV antigen in the respiratory tract and scanning electron microscopic alterations of the tracheal epithelium until 36 days post infection (p.i.). In both groups, clinical signs were minimal, restricted to the upper respiratory tract and consisted of cough elicited by tracheal compression between 3 and 7 days p.i. Microscopically, inflammatory and degenerative lesions were observed in the trachea and less frequently in the nasal cavity; bronchiolitis or interstitial pneumonia was not demonstrated. By immunocytochemistry, CPIV antigen was demonstrated in tracheal epithelial cells, whereas nasal cavity, bronchi, bronchioles and lung were devoid of viral antigen. Ferrets given CPIV alone developed a minimal lymphocytic tracheitis with minimal loss of cilia and CPIV antigen was observed only 4 days p.i. 17 days p.i., normal epithelial organization and ciliary reappearance was reestablished. Ferrets treated with cyclophosphamide and infected with CPIV exhibited mild to moderate histological lesions as above with similar scanning electron microscopic changes until 36 p.i. Tracheal lesions consisted of intraepithelial and submucosal infiltration of lymphocytes and macrophages, focal epithelial hyperplasia and multifocal loss of cilia. In addition, mild and transient neutrophilic infiltration was observed. In immunosuppressed ferrets, viral antigen expression was prominent and demonstrated 4 and 8 days p.i. These data suggest that ferrets are susceptible to aerosol CPIV infection.
Similar articles
-
In vitro cytopathogenicity and in vivo virulence of two strains of canine parainfluenza virus.Vet Pathol. 1991 Jul;28(4):324-31. doi: 10.1177/030098589102800409. Vet Pathol. 1991. PMID: 1659020
-
Role of canine parainfluenza virus and Bordetella bronchiseptica in kennel cough.Am J Vet Res. 1984 Sep;45(9):1862-6. Am J Vet Res. 1984. PMID: 6093651
-
Pathogenesis of bronchiolitis and pneumonia induced in neonatal and weanling rats by parainfluenza (Sendai) virus.Am J Pathol. 1987 Nov;129(2):277-86. Am J Pathol. 1987. PMID: 2823613 Free PMC article.
-
Canine parainfluenza virus-induced encephalitis in ferrets.J Comp Pathol. 1989 Jan;100(1):67-76. doi: 10.1016/0021-9975(89)90091-1. J Comp Pathol. 1989. PMID: 2537347
-
Molecular biology of canine parainfluenza virus V protein and its potential applications in tumor immunotherapy.Front Microbiol. 2023 Dec 20;14:1282112. doi: 10.3389/fmicb.2023.1282112. eCollection 2023. Front Microbiol. 2023. PMID: 38173672 Free PMC article. Review.
Cited by
-
Experimental infection of dromedaries with Middle East respiratory syndrome-Coronavirus is accompanied by massive ciliary loss and depletion of the cell surface receptor dipeptidyl peptidase 4.Sci Rep. 2018 Jun 27;8(1):9778. doi: 10.1038/s41598-018-28109-2. Sci Rep. 2018. PMID: 29950581 Free PMC article.
-
Alston Virus, a Novel Paramyxovirus Isolated from Bats Causes Upper Respiratory Tract Infection in Experimentally Challenged Ferrets.Viruses. 2018 Nov 28;10(12):675. doi: 10.3390/v10120675. Viruses. 2018. PMID: 30487438 Free PMC article.
-
Isolation and Molecular Characterization of Swine Parainfluenza Virus 5 from Piglets Co-Infected with PEDV.Vet Sci. 2025 Jul 18;12(7):676. doi: 10.3390/vetsci12070676. Vet Sci. 2025. PMID: 40711336 Free PMC article.
-
Molecular detection and whole genome characterization of Canine Parainfluenza type 5 in Thailand.Sci Rep. 2021 Feb 16;11(1):3866. doi: 10.1038/s41598-021-83323-9. Sci Rep. 2021. PMID: 33594165 Free PMC article.
-
Ferret models of viral pathogenesis.Virology. 2015 May;479-480:259-70. doi: 10.1016/j.virol.2015.03.017. Epub 2015 Mar 26. Virology. 2015. PMID: 25816764 Free PMC article. Review.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources