Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Jun;72(6):2064-8.
doi: 10.1073/pnas.72.6.2064.

Involvement of nicotinamide adenine dinucleotide in the action of cholera toxin in vitro

D M Gill

Involvement of nicotinamide adenine dinucleotide in the action of cholera toxin in vitro

D M Gill. Proc Natl Acad Sci U S A. 1975 Jun.

Abstract

NAD is a necessary cofactor for the activation of adenylate cyclase (ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1) by cholera toxin. Lysates of certain types of cell that hydrolyze their endogenous store of NAD after cell disruption respond poorly or not at all to cholera toxin. Lysates of pigeon erythrocytes, which lack enzymes that degrade NAD, provide a convenient and reproducible system for assaying the activity of cholera toxin in vitro and allow investigation of the mechanism of action of the toxin upon broken cells.

PubMed Disclaimer

References

    1. Biochim Biophys Acta. 1955 Oct;18(2):296 - PubMed
    1. J Exp Med. 1967 Nov 1;126(5):913-21 - PubMed
    1. Anal Biochem. 1971 Feb;39(2):441-53 - PubMed
    1. J Biol Chem. 1972 Sep 25;247(18):5964-71 - PubMed

Publication types

LinkOut - more resources