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. 1975 Jun;72(6):2413-7.
doi: 10.1073/pnas.72.6.2413.

Viral and cellular DNA synthesis in nuclei from human lymphocytes transformed by Epstein-Barr virus

W C Benz, J L Strominger

Viral and cellular DNA synthesis in nuclei from human lymphocytes transformed by Epstein-Barr virus

W C Benz et al. Proc Natl Acad Sci U S A. 1975 Jun.

Abstract

A DNA-synthesizing system in vitro, using nuclei prepared by treatment of human lymphocytes with the detergent Brij 58, was developed. Nuclei from cultured lymphocytes synthesized DNA for as long as 5 hr, and required ATP, deoxynucleoside triphosphates, magnesium, and a calcium chelator. In nuclei from a partially synchronized line of cultured lymphocytes carrying several hundred copies of the Epstein-Barr viral genome, synthesis in vitro was predominately viral in early S phase and cellular in late S phase. These and other data suggested that the DNA synthesis observed in vitro was predominately replicative.

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