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. 1991 Dec 11;108(2):141-4.
doi: 10.1007/BF00233118.

An assay for glucose 6-phosphatase based on the formation of glucose

C Bublitz  1
Affiliations

An assay for glucose 6-phosphatase based on the formation of glucose

C Bublitz. Mol Cell Biochem. .

Abstract

A rapid, convenient method for the assay of glucose 6-phosphatase dependent on the removal of radioactive substrate from radioactive product by Dowex 2 fluoride is described. The enzymatic reaction is stopped by the addition of an ethanolic slurry of the resin. After the tubes are shaken, the radioactivity of glucose in the clarified supernatant layer is measured. The release of glucose is directly proportional to time and enzyme concentration. Detergents do not interfere with the method.

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