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. 2006 May 22;94(10):1492-5.
doi: 10.1038/sj.bjc.6603117.

Cell-free and cell-bound circulating DNA in breast tumours: DNA quantification and analysis of tumour-related gene methylation

T E Skvortsova  1 , E Y Rykova, S N Tamkovich, O E Bryzgunova, A V Starikov, N P Kuznetsova, V V Vlassov, P P Laktionov
Affiliations

Cell-free and cell-bound circulating DNA in breast tumours: DNA quantification and analysis of tumour-related gene methylation

T E Skvortsova et al. Br J Cancer. .

Abstract

Tumour development is characterised by the increased circulating DNA (cirDNA) concentration and by tumour-related changes in blood plasma DNA. Concentration of cirDNA and methylation of RARbeta2, RASSF1A and HIC-1 gene promoters were investigated in cell-free and cell-surface-bound fractions from healthy donors, patients with breast cancer, and patients with breast fibroadenoma. Tumour development was shown to lead to significant changes in the distribution of cirDNA between cell-free and cell-surface-bound fractions. Analysis of RARbeta2 and RASSF1A methylation in the total cirDNA provides 95% diagnostic coverage in breast cancer patients, 60% in patients with benign lesions, and is without false-positive results in healthy women. Results of the study indicate that methylation-specific PCR of RARbeta2 and RASSF1A genes based on the total cirDNA combined with the quantitative analysis of cirDNA distribution between cell-bound and cell-free fractions in blood provide the sensitive and accurate detection and discrimination of malignant and benign breast tumours.

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Figures

Figure 1
Figure 1
Concentrations of circulating DNA in plasma (Plasma) and cell-surface-bound DNA (CSB) in blood of healthy women (Health), breast cancer patients (Cancer) and patients with breast fibroadenoma (Fibroadenoma).
See this image and copyright information in PMC

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