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. 2006 May 1;31(9):1292-4.
doi: 10.1364/ol.31.001292.

Broadly tunable dual-wavelength light source for coherent anti-Stokes Raman scattering microscopy

Feruz Ganikhanov  1 , Silvia Carrasco, X Sunney Xie, Mordechai Katz, Wolfgang Seitz, Daniel Kopf
Affiliations

Broadly tunable dual-wavelength light source for coherent anti-Stokes Raman scattering microscopy

Feruz Ganikhanov et al. Opt Lett. .

Abstract

The signal and idler beams from a picosecond, synchronously pumped optical parametric oscillator (OPO) provide the two colors necessary for coherent anti-Stokes Raman scattering (CARS) microscopy. The OPO provides a continuously tunable frequency difference between the two beams over a broad range of Raman shifts (100-3700 cm(-1)) by varying the temperature of a single nonlinear crystal. The near-infrared output (900-1300 nm) allows for deep penetration into thick samples and reduced nonlinear photodamage. Applications of this light source to in vivo cell and ex vivo tissue imaging are demonstrated.

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