[Characteristics of poliovirus strains isolated in Poland during 1981-1989 using monoclonal antibodies and determination of nucleotide sequence in viral RNA]

Abstract

In this study currently used methods for analysis of poliovirus with monoclonal antibodies and determination of nucleotide sequence in viral RNA are presented. Twenty one strains of polioviruses isolated in Poland between 1981 and 1989 were tested. Nineteen of these strains were determined as to be derived from attenuated strains, including 10 strains isolated from 9 poliomyelitis patients. Two strains, one of type 1 isolated in 1984 and one of type 2 isolated in 1982 from children with meningitis were determined to be wild strains. The results obtained with monoclonal antibodies were confirmed by partial nucleotide sequence by dideoxy primer extension method. Analysis of poliovirus strains circulating in Poland in last 15 years suggest that the elimination of wild strains was achieved and sporadic isolation of wild strains is due most probably to imported strains.