Coordinate Gene Response to Salt Stress in Lophopyrum elongatum

Abstract

Lophopyrum elongatum is a highly salt-tolerant relative of wheat. A previous study showed that the abundance of a number of mRNA species is enhanced or reduced in the roots of the L. elongatum x Triticum aestivum amphiploid by salt stress. Eleven genes with enhanced expression in the roots of salt-stressed L. elongatum plants have been cloned as cDNAs. The clones were used as probes to characterize temporal expression of these genes in roots after initiation of salt (250 mm NaCl) stress. All 11 genes are induced within 2 h after exposure to 250 mm NaCl and reached peak expression after 6 h. The decline of gene expression distinguished two groups, one in which mRNA concentrations returned to basal levels by 24 h and the other in which this occurred between 3 and 7 d. One of the 11 clones was found to be homologous to a multigene family of abscisic acid-induced genes, rab and dhn, identified in other species. We suggest that the coordinate expression of this large number of genes reflects the existence of a highly specific early response to salt stress. We refer to this response as the "early salt stress response."