Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Nov;100(3):1591-4.
doi: 10.1104/pp.100.3.1591.

Detection of a Cytosolic Glutamine Synthetase in Leaves of Nicotiana tabacum L. by Immunocytochemical Methods

H Carvalho  1 , S Pereira, C Sunkel, R Salema
Affiliations

Detection of a Cytosolic Glutamine Synthetase in Leaves of Nicotiana tabacum L. by Immunocytochemical Methods

H Carvalho et al. Plant Physiol. 1992 Nov.

Abstract

Two glutamine synthetase (GS) polypeptides (44 and 39 kD) were immunodetected on western blots of leaf extracts from tobacco (Nicotiana tabacum L.), a plant that has been reported to contain only chloroplast GS in the leaves. By immunocytochemical methods, we confirmed the localization of GS in the cytosol of cells in the vascular tissue and in the chloroplasts of mesophyll cells.

PubMed Disclaimer

References

    1. Plant Physiol. 1984 Feb;74(2):448-50 - PubMed
    1. Plant Physiol. 1987 Jun;84(2):366-73 - PubMed
    1. Plant Physiol. 1983 May;72(1):22-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1990 May;87(9):3459-63 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed

LinkOut - more resources