Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1966 Feb;41(2):214-20.
doi: 10.1104/pp.41.2.214.

Purification and properties of apple fruit malic enzyme

D R Dilley  1
Affiliations

Purification and properties of apple fruit malic enzyme

D R Dilley. Plant Physiol. 1966 Feb.

Abstract

Malic enzyme was isolated and purified from mature apple fruits (Malus sylvestris, Miller) by utilizing procedures probably applicable to other soluble enzymes in this and similar tissues.The physical properties of apple fruit malic enzyme are similar to those reported for malic enzyme from other plant and animal sources. It is specific for l-malate, TPN and requires a divalent cation for activity. In contrast to the pigeon liver enzyme, supplemental TPN is not required for oxalacetic decarboxylase activity of the fruit enzyme. The pH optimum of the malic enzyme varied with the l-malate concentration and the nature of the divalent cation present. d-Malate activated the oxidation of l-malate at rate-limiting concentrations.

PubMed Disclaimer

References

    1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
    1. Plant Physiol. 1962 Jan;37(1):1-7 - PubMed