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. 1967 Jul;42(7):959-67.
doi: 10.1104/pp.42.7.959.

Relatedness Among Plants as Measured by the DNA-Agar Technique

A J Bendich  1 , E T Bolton
Affiliations

Relatedness Among Plants as Measured by the DNA-Agar Technique

A J Bendich et al. Plant Physiol. 1967 Jul.

Abstract

An improved method for extraction of plant DNA is described. Quantitative species comparisons based on DNA-DNA hybridization are reported for several members of the family Leguminosae and for barley, wheat and rye. A maximum of about 10% homology in DNA polynucleotide sequences is found between monocotyledons and dicotyledons tested, whereas 20 to 90% homology is observed within a family. Species compared using a DNA fraction enriched for redundant polynucleotide sequences generally appear to be more closely related than when whole DNA is used. DNA-DNA hybridization may be useful in systematic and evolutionary study of plants, and also as a possible screening procedure for interfertility of species.

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References

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