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. 1967 Sep;42(9):1179-86.
doi: 10.1104/pp.42.9.1179.

In Vitro Protein Synthesis by Plastids of Phaseolus vulgaris. I. Localization of Activity in the Chloroplasts of a Chloroplast Containing Fraction from Developing Leaves

F Parenti  1 , M M Margulies
Affiliations

In Vitro Protein Synthesis by Plastids of Phaseolus vulgaris. I. Localization of Activity in the Chloroplasts of a Chloroplast Containing Fraction from Developing Leaves

F Parenti et al. Plant Physiol. 1967 Sep.

Abstract

The incorporation of uniformly labeled leucine-(14)C into protein by a chloroplast containing fraction from developing primary leaves of bean is reported. Chloroplasts, obtained from week old plants grown in darkness, and then illuminated with white light for 12 hours, were shown to be the principal sites of incorporating activity. Incorporation may continue for 2 hours. Rates of up to 50 mumumole leucine incorporated per mg protein per hour are observed when a 1 hour assay period is used. Incorporation is only partially sensitive to ribonuclease.

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References

    1. J Mol Biol. 1966 Jun;17(2):470-87 - PubMed
    1. Biochim Biophys Acta. 1965 Mar 15;95:474-85 - PubMed
    1. Biochim Biophys Acta. 1963 Oct 15;76:319-21 - PubMed
    1. Biochim Biophys Acta. 1963 Oct 15;76:286-92 - PubMed
    1. Plant Physiol. 1964 Jul;39(4):579-85 - PubMed

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