Structure and function of tomato leaf chloroplasts during ammonium toxicity

Abstract

Ammonium toxicity resulted in morphological modifications of tomato leaf chloroplasts. The chloroplasts, which are normally flattened around the protoplast periphery, became ellipsoidally rounded and dispersed through the protoplasm. The first apparent effect of plastid degradation was development of many vesicles from the fretwork. Later the grana lamellae swelled, and some disappeared. Eventually, distinct grana could not be detected.Ammonium accumulation, chlorophyll loss, and photosynthetic decrease occurred simultaneously. Initial changes in these processes preceded the detection of modifications of fine structure; however, each continued with further breakdown of the chloroplasts.