Endogenous gibberellins of a radiation induced single gene dwarf mutant of bean

Abstract

The distribution of endogenous gibberellins in Dwarf-1, a single gene dwarf mutant of Mexico 80-R red beans, was studied. Parallel extraction and fractionation of seeds of this mutant and those of a normal homozygous line followed by thin layer chromatography and bioassays using Rumex obtusifolius, wheat seed endosperm and dwarf bean plants revealed that a stem elongation control factor was contained in the non-acidic fraction from normal, but not from Dwarf-1, seeds. It was concluded that the single gene mutation causes a block either in gibberellin precursor formation or in production of a non-acidic fraction gibberellin-like substance.