A detailed evaluation of the possible contribution of bacteria to radioactive precursor incorporation into nucleic acids of plant tissues

Abstract

An investigation of the possible contribution of bacteria to the labeling patterns of soybean seedling nucleic acid was made. The results using sucrose gradient, MAK column, and acrylamide gel electrophoretic fractionation together with base composition analyses of nucleic acid preparations show that contaminating bacteria do not contribute to the incorporation of (32)P-orthophosphate into the RNA of excised hypocotyl or soybean root tip. Sterile, non-sterile, and CM-treated soybean hypocotyl synthesize D-RNA to the same extent. The contaminating bacteria do not synthesize an AMP-rich RNA. The G-C rich (32)P-DNA component of the soybean tissues used in these studied results, at least primarily, from the incorporation by contaminating bacteria. CM can be used successfully to eliminate the contribution of bacteria to the labeling of nucleic acids by etiolated plant tissues. Bacterial counts, although valuable, are not sufficient to determine if contaminating bacteria will significantly contribute to nucleic acid labeling in plants.