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. 1968 Sep;43(9):1389-94.
doi: 10.1104/pp.43.9.1389.

Gas-liquid chromatography of trimethylsilyl derivatives of abscisic Acid and other plant hormones

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Gas-liquid chromatography of trimethylsilyl derivatives of abscisic Acid and other plant hormones

L A Davis et al. Plant Physiol. 1968 Sep.

Abstract

This paper describes a new method for qualitative and quantitative assay of abscisic acid and other acidic plant hormones, such as indoleacetic acid and the gibberellins, by the gas-liquid chromatography of their trimethylsilyl derivatives. Interfering substances in plant extracts were largely removed by preliminary column chromatography with carbon-celite and elution of the abscisic acid with 60% acetone, permitting direct determination of abscisic acid by gas-liquid chromatography using a flame ionization detector. (A level of 0.65 mg/kg fr wt was found.) This method enables measurement of amounts of abscisic acid as low as 0.025 mug. In impure samples collected by gas-liquid chromatography the abscisic acid recovered could be measured quantitatively by use of its ultraviolet absorption maximum at 260 mmu.

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References

    1. Plant Physiol. 1964 Sep;39(5):836-42 - PubMed
    1. Anal Biochem. 1966 Jul;16(1):36-52 - PubMed
    1. Anal Biochem. 1967 Sep;20(3):484-94 - PubMed

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