The isolation of spinach chloroplasts in pyrophosphate media
- PMID: 16656930
- PMCID: PMC1087032
- DOI: 10.1104/pp.43.9.1415
The isolation of spinach chloroplasts in pyrophosphate media
Abstract
A simplified procedure (involving disruption in sorbitol-pyrophosphate mixtures) permits the separation of spinach chloroplasts which retain the ability to catalyze the photosynthetic assimilation of carbon dioxide and its associated oxygen evolution.
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