Effect of cycloheximide, gamma irradiation, and phosphorus deficiency on root pressure exudation in tobacco

Abstract

Cycloheximide drastically reduced the rate of root pressure exudation in detopped tobacco (Nicotiana tabacum L.), and the effect was more pronounced for nitrate salts in the external solution than for some other salts or when the roots were in water. Diurnal periodicity was greatly decreased, and its phase was changed. Effects began within an hour. Cation uptake was reduced by relatively low levels of cycloheximide. The effects of cycloheximide seemed to be reversible. Mild phosphorus deficiency resulted in decreased exudation rates from detopped tobacco and in no response to nitrate. Periodicity of exudation was not greatly affected by phosphorus deficiency, however. Gamma ray irradiation with a (60)Co source at levels (up to 40-50 kiloroentgens) which are considered disruptive of moderately large molecules had relatively little effect on the exudation rate. Higher levels of irradiation, which disrupt most protein molecules, decreased exudation and obscured periodicity. The results indicate either that new protein (or peptide) synthesis is needed for the rapid nitrate transport or that the deficiency and inhibitor disrupt cellular membranes. Phosphorus deficiency increased the sensitivity of the plants to inhibition by irradiation of the exudation process.