Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1971 Feb;47(2):217-21.
doi: 10.1104/pp.47.2.217.

Isolation of Intact Chloroplasts from Euglena gracilis by Zonal Centrifugation

A Vasconcelos  1 , M Pollack, L R Mendiola, H P Hoffmann, D H Brown, C A Price
Affiliations

Isolation of Intact Chloroplasts from Euglena gracilis by Zonal Centrifugation

A Vasconcelos et al. Plant Physiol. 1971 Feb.

Abstract

Chloroplasts were separated from Euglena gracilis by zonal centrifugation at low speed in density gradients of Ficoll or dextran. The chloroplasts were intact by the criteria of ultrastructure and their content of ribulose diphosphate carboxylase and soluble protein. The chloroplasts also contained ribosomes and ribosomal RNA uncontaminated by the corresponding cytoplasmic particles.

PubMed Disclaimer

References

    1. Biochim Biophys Acta. 1964 May 25;79:637-9 - PubMed
    1. Plant Physiol. 1967 Jan;42(1):1-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1095-101 - PubMed
    1. Science. 1966 Sep 30;153(3744):1661-3 - PubMed
    1. Biochemistry. 1968 Feb;7(2):668-74 - PubMed

LinkOut - more resources