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. 1974 Mar;53(3):449-52.
doi: 10.1104/pp.53.3.449.

Characteristics of an Acid protease from maize endosperm

B M Harvey  1 , A Oaks
Affiliations

Characteristics of an Acid protease from maize endosperm

B M Harvey et al. Plant Physiol. 1974 Mar.

Abstract

An assay has been developed to measure protease activity in endosperm extracts of maize seeds. With hemoglobin as substrate, the enzyme(s) has a pH optimum of 3.8 and a temperature optimum of 46 C. It also degrades gliadin, edestin, bovine serum albumin, and partially hydrolyzed zein and glutelin under standard assay conditions. The enzyme(s) has endopeptidase activity with all substrates tested. When undenatured zein and glutelin are suspended in an agar gel, both are efficiently degraded. Using this assay, the protease activity increases from day 3 to day 8 after inhibition and then declines.

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References

    1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
    1. Plant Physiol. 1967 Nov;42(11):1596-600 - PubMed
    1. Plant Physiol. 1974 Mar;53(3):453-7 - PubMed
    1. Plant Physiol. 1969 Nov;44(11):1488-98 - PubMed

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