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. 1974 May;53(5):688-90.
doi: 10.1104/pp.53.5.688.

Use of protein in extraction and stabilization of nitrate reductase

L E Schrader  1 , D A Cataldo, D M Peterson
Affiliations

Use of protein in extraction and stabilization of nitrate reductase

L E Schrader et al. Plant Physiol. 1974 May.

Abstract

The in vitro instability of nitrate reductase (EC 1.6.6.1) activity from leaves of several species of higher plants was investigated. Decay of activity was exponential with time, suggesting that an enzyme-catalyzed reaction was involved. The rate of decay of nitrate reductase activity increased as leaf age increased in all species studied. Activity was relatively stable in certain genotypes of Zea mays L., but extremely unstable in others. In all genotypes of Avena sativa L. and Nicotiana tabacum L. studied, nitrate reductase was unstable. Addition of 3% (w/v) bovine serum albumin or casein to extraction media prevented or retarded the decay of nitrate reductase activity for several hours. In addition, the presence of bovine serum albumin or casein in the enzyme homogenate markedly increased nitrate reductase activity (up to 15-fold), especially in older leaf tissue.

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References

    1. Plant Physiol. 1965 Jul;40(4):691-8 - PubMed
    1. Biochim Biophys Acta. 1972 Dec 14;283(3):505-12 - PubMed
    1. Proc Natl Acad Sci U S A. 1969 Mar;62(3):785-92 - PubMed

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