Concentrations of Indole-3-acetic Acid and Its Esters in Avena and Zea
- PMID: 16658870
- PMCID: PMC367393
- DOI: 10.1104/pp.54.3.257
Concentrations of Indole-3-acetic Acid and Its Esters in Avena and Zea
Abstract
An isotope-dilution method has been developed for the assay of free indole-3-acetic acid and ester indole-3-acetic acid as measured by indole-3-acetic acid liberated by mild alkaline hydrolysis. Application of this method to seedlings of Avena sativa and Zea mays indicates the upper limit of free indole-3-acetic acid in Avena to be about 16 mug per kg and in Zea, about 24 mug. The amount of 1 n alkali-labile indole-3-acetic acid in Zea is about 330 mug per kg and there is very little 1 n alkali-labile IAA in Avena. A chemical characterization of the indole-3-acetic acid of Avena and a confirmation of the chemical characterization of the indole-3-acetic acid of Zea is presented.
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