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. 1974 Nov;54(5):791-3.
doi: 10.1104/pp.54.5.791.

Microenvironmental manipulation of the observed michaelis constant of ribulose diphosphate carboxylase

L E Anderson  1
Affiliations

Microenvironmental manipulation of the observed michaelis constant of ribulose diphosphate carboxylase

L E Anderson. Plant Physiol. 1974 Nov.

Abstract

Urease and ribulose 1, 5-diphosphate carboxylase can be bound to Sepharose to give an immobilized two-enzyme system which catalyzes the reaction urea --> H(2)CO(3) --> phosphoglyceric acid. The observed Km of the system for urea approaches the lower value for urease when carboxylase levels on the gel exceed urease levels. If a similar system operates in the chloroplast, the high Km (H(2)CO(3)) of ribulose 1,5-diphosphate carboxylase may not be metabolically significant.

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References

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