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. 1975 Nov;56(5):665-70.
doi: 10.1104/pp.56.5.665.

Studies with detached lupin root nodules in culture: I. Maintenance and induction of acetylene reduction activity

W D Sutton  1 , N M Jepsen
Affiliations

Studies with detached lupin root nodules in culture: I. Maintenance and induction of acetylene reduction activity

W D Sutton et al. Plant Physiol. 1975 Nov.

Abstract

A method has been developed for culturing detached nitrogen-fixing root nodules of lupin (Lupinus angustifolius L.) on a simple nutrient medium. Under the best conditions devised, the acetylene reduction activity of mature detached nodules was maintained at 10 to 25 nmoles of ethylene hr(-1) mg(-1) fresh weight for 3 days. Under the same culture conditions, immature nodules increased their acetylene reduction activity from 0.01 nmole or less to about 1 nmole hr(-1) mg(-1) fresh weight.

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