Ozone-induced Loss of Intracellular Potassium Ion from Chlorella sorokiniana

Abstract

The unicellular algae Chlorella sorokiniana was used as a model system to investigate the interaction of ozone with plant cell membranes. Ozone induces K(+) leakage from Chlorella sorokiniana similar to the electrolytic loss observed from many higher plants under stressful conditions. The kinetics of this leakage indicate that ozone initially interacts reversibly (within sec) with sites on membranes allowing a passive efflux of K(+). This efflux ceases within minutes after the ozone stress is removed. This return to normal efflux is very temperature dependent. High intracellular osmolarity seems to be an important criterion of susceptibility to ozone injury in this model system, since rates of ozone-induced K(+) leakage are less when the external osmotic potential is decreased by suspension of the cells in mannitol. Cell interaction with ozone is further complicated by a saturating-type dependence of the K(+) efflux upon ozone concentration within the medium.