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. 1975 Dec;56(6):853-5.
doi: 10.1104/pp.56.6.853.

Purification of NADH-Nitrate Reductase by Affinity Chromatography

L P Solomonson  1
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Purification of NADH-Nitrate Reductase by Affinity Chromatography

L P Solomonson. Plant Physiol. 1975 Dec.

Abstract

ASSIMILATORY NITRATE REDUCTASE (NADH: nitrate oxidoreductase, EC 1.6.6.1) from Chlorella vulgaris has been purified to electrophoretic homogeneity with an overall yield of 60% by a procedure that utilizes blue dextran-agarose as an affinity column. Nitrate reductase binds to blue dextran and is not eluted in the presence of high ionic strength buffer but is rapidly eluted in the presence of mumolar concentrations of NADH.

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References

    1. J Biol Chem. 1969 Jun 10;244(11):2870-82 - PubMed
    1. Biochim Biophys Acta. 1972 Aug 17;275(2):276-8 - PubMed
    1. Biochim Biophys Acta. 1972 Jun 23;267(3):544-57 - PubMed
    1. Arch Biochem Biophys. 1974 Jan;160(1):279-84 - PubMed
    1. Eur J Biochem. 1971 Sep 13;22(1):40-5 - PubMed

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