Effect of glycidate on glycolate formation and photosynthesis in isolated spinach chloroplasts

Abstract

Glycidate (2,3-epoxypropionate) increased CO(2) photoassimilation in intact spinach (Spinacia oleracea L.) chloroplasts in the presence of various inhibitors of photosynthesis, including O(2), arsenite, azide, iodo-acetamide, and carbonylcyanide 3-chlorophenylhydrazone. Although the mechanism by which glycidate enhances photosynthesis is obscure, the stimulatory effect cannot be ascribed to either an inhibition of glycolate formation, a specific interaction with the O(2) inhibition of photosynthesis, or a direct effect on the ribulose 1,5-bisphosphate carboxylase (EC 4.1.1.39) reaction. The lack of a differential effect of glycidate on photosynthesis and glycolate formation in the isolated chloroplast was confirmed in whole leaf studies by the CO(2) compensation concentration assay. These results are at variance with the report that glycidate stimulates net photosynthesis in tobacco leaf disks by irreversibly inhibiting glycolate formation and thus photorespiration (Zelitch, I., 1974, Arch. Biochem. Biophys. 163: 367-377).