Isotope Discrimination by Ribulose 1,5-Diphosphate Carboxylase: No Effect of Temperature or HCO(3) Concentration
- PMID: 16659531
- PMCID: PMC542077
- DOI: 10.1104/pp.57.4.580
Isotope Discrimination by Ribulose 1,5-Diphosphate Carboxylase: No Effect of Temperature or HCO(3) Concentration
Abstract
Carbon 13 isotope discrimination by ribulose 1,5-diphosphate carboxylase from soybean (Glycine max [Merr.] cv. Amsoy) was studied as a function of temperature, bicarbonate concentration, and pH. None of these factors affected the degree of discrimination against (13)C. The average delta(13)C was -28.3%, a value close to that found for whole C(3) plants. The zero temperature response observed here with ribulose 1,5-diphosphate carboxylase corroborates data from whole plants. The lack of effect of bicarbonate concentration on discrimination is consistent with both current theories of alternate forms of carboxylase.
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