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. 1977 Jun;59(6):1178-81.
doi: 10.1104/pp.59.6.1178.

Strong binding of cytochrome C on the envelope of spinach chloroplasts

M Neuburger  1 , J Joyard, R Douce
Affiliations

Strong binding of cytochrome C on the envelope of spinach chloroplasts

M Neuburger et al. Plant Physiol. 1977 Jun.

Abstract

Yeast cationic ferricytochrome c was able to bind to the spinach (Spinacia oleracea) chloroplast envelope with a low affinity (Kd = 1.1 mum). The total amount of low affinity binding sites was of the order of 50 nmol cytochrome c mg(-1) protein. We gave the evidence that binding of ferricytochrome c to the envelope was electrostatic and that the envelope membranes were strongly negatively charged. Addition of yeast ferricytochrome c to a preparation of intact washed chloroplasts (class I) induced a strong agglutination of chloroplasts.

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References

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