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. 1977 Nov;60(5):670-4.
doi: 10.1104/pp.60.5.670.

Cysteinyl-tRNA Synthetase from Phaseolus aureus: Purification and Properties

J N Burnell  1
Affiliations

Cysteinyl-tRNA Synthetase from Phaseolus aureus: Purification and Properties

J N Burnell. Plant Physiol. 1977 Nov.

Abstract

l-Cysteinyl-tRNA synthetase (EC 6.1.1.16) from Phaseolus aureus was purified approximately 300-fold and was free of contaminating aminoacyl-tRNA synthetases. Optimum assay conditions were determined and substrate specificity and inhibitor properties were investigated using the ATP-PPi exchange reaction. The Km values for l-cysteine, ATP, and PPi were 6.20 x 10(-5)m, 1.15 x 10(-3)m, and 1 x 10(-3)m, respectively. Both l-selenocysteine (Km = 5 x 10(-5)m) and alpha-l-aminobutyric acid (Km = 1 x 10(-2)m) acted as alternative substrates of the purified cysteinyl-tRNA synthetase. The enzyme was sensitive to sulfhydryl group reagents; it was inhibited by sulfide, 0-acetylserine, and reduced glutathione.

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References

    1. FEBS Lett. 1972 Jul 15;24(1):60-2 - PubMed
    1. J Biol Chem. 1969 Jun 25;244(12):3210-6 - PubMed
    1. Plant Physiol. 1976 Sep;58(3):248-52 - PubMed
    1. Adv Enzymol Relat Areas Mol Biol. 1967;29:89-163 - PubMed
    1. Ann Inst Pasteur (Paris). 1958 Nov;95(5):615-36 - PubMed

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