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. 1978 Feb;61(2):254-8.
doi: 10.1104/pp.61.2.254.

Indole-3-acetic Acid Levels of Plant Tissue as Determined by a New High Performance Liquid Chromatographic Method

P B Sweetser  1 , D G Swartzfager
Affiliations

Indole-3-acetic Acid Levels of Plant Tissue as Determined by a New High Performance Liquid Chromatographic Method

P B Sweetser et al. Plant Physiol. 1978 Feb.

Abstract

A method for the analysis of indole-3-acetic acid (IAA) in plant extracts has been developed based on high performance liquid chromatography separation of IAA on a microparticulate strong anion exchange column followed by quantitation with two selective detectors: an electrochemical, carbon paste amperometric detector and/or a fluorescence detector. The detection limit for IAA is less than 1 nanogram with the fluorescence detector and less than 50 picograms with the electrochemical detector.The IAA levels are reported for various tissues of wheat, pinto beans, soybeans, cotton, and corn.

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References

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