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. 1978 Dec;62(6):987-90.
doi: 10.1104/pp.62.6.987.

Nitrate Utilization by the Diatom Skeletonema costatum: I. Kinetics of Nitrate Uptake

J L Serra  1 , M J Llama, E Cadenas
Affiliations

Nitrate Utilization by the Diatom Skeletonema costatum: I. Kinetics of Nitrate Uptake

J L Serra et al. Plant Physiol. 1978 Dec.

Abstract

Nitrate uptake has been studied in nitrogen-deficient cells of the marine diatom Skeletonema costatum. When these cells are incubated in the presence of nitrate, this ion is quickly taken up from the medium, and nitrite is excreted by the cells. Nitrite is excreted following classical saturation kinetics, its rate being independent of nitrate concentration in the incubation medium for nitrate concentration values higher than 3 micromolar. Nitrate uptake shows mixed-transfer kinetics, which can be attributed to the simultaneous contributions of mediated and diffusion transfer. Cycloheximide and p-hydroxymercuribenzoate inhibit the carrier-mediated contribution to nitrate uptake, without affecting the diffusion component. When cells are preincubated with nitrate, the net nitrogen uptake is increased.

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References

    1. Plant Physiol. 1975 Nov;56(5):692-5 - PubMed
    1. Science. 1971 Mar 12;171(3975):1008-13 - PubMed
    1. Plant Physiol. 1976 Jan;57(1):55-8 - PubMed
    1. Plant Physiol. 1973 Oct;52(4):362-7 - PubMed
    1. Plant Physiol. 1973 Jan;51(1):120-7 - PubMed

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