Cysteinyl-tRNA Synthetase from Astragalus Species
- PMID: 16660863
- PMCID: PMC542976
- DOI: 10.1104/pp.63.6.1095
Cysteinyl-tRNA Synthetase from Astragalus Species
Abstract
l-Cysteinyl-tRNA synthetases (EC 6.1.1.16) from four Astragalus species were partially purified. The substrate specificities of the cysteinyl-tRNA synthetase from three selenium accumulator species (A. crotalariae, A bisulcatus, and A. racemosus) were compared with those from two nonaccumulator species (A. lentigenosus and Phaseolus aureus). All species had similar K(m) values for cysteine, selenocysteine, and alpha-aminobutyric acid except A. bisulcatus which failed to use selenocysteine as a substrate and which had a K(m) for cysteine four times greater than the K(m) values for other species.
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