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. 1979 Jun;63(6):1212-4.
doi: 10.1104/pp.63.6.1212.

Isolation of Protoplasts and Chloroplasts from Flag Leaves of Triticum aestivum L

R C Leegood  1 , D A Walker
Affiliations

Isolation of Protoplasts and Chloroplasts from Flag Leaves of Triticum aestivum L

R C Leegood et al. Plant Physiol. 1979 Jun.

Abstract

Intact protoplasts and chloroplasts have been isolated from mature flag leaves of wheat (Triticum aestivum L.). Both showed high rates of photosynthesis, the best of which equaled those observed in the parent tissue (greater than 150 micromoles O(2) per milligram chlorophyll per hour). The presence of ethylenediaminetetraacetate and an alkaline medium (pH 8.4) were required in the isolation and assay for the achievement of maximum rates of photosynthesis by chloroplasts. Photosynthesis by isolated chloroplasts was inhibited at very low concentrations of external orthophosphate.

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References

    1. Plant Physiol. 1977 Mar;59(3):422-7 - PubMed
    1. Plant Physiol. 1978 Aug;62(2):313-9 - PubMed
    1. Arch Biochem Biophys. 1978 Oct;190(2):421-33 - PubMed
    1. Biochim Biophys Acta. 1973 Dec 14;325(3):596-604 - PubMed

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